| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021635 |
| Chromosome: | chromosome 15 |
| Location: | 1223388 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g801721 | NCL61 | Nuclear Control of chloroplast-Like 61; (1 of 19) PTHR21228//PTHR21228:SF20 - FAST LEU-RICH DOMAIN-CONTAINING // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCCGCTCAGGAGGCCTACCTGCTGCAGCAGCTGCAGGATCTGCTAT |
| Internal bar code: | AAAATTGGAACAGGGTTCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2250 |
| LEAP-Seq percent confirming: | 12.9032 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCACCTGCTTCTCAGAG |
| Suggested primer 2: | CTTGTTGCACAGGCCCTTTG |