Insertion junction: CLIP2.021661_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.021661
Chromosome:chromosome 3
Location:551336
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145227 (1 of 1) K12199 - vacuolar protein sorting-associated protein VTA1 (VTA1, LIP5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCTCCAGCGACTGGGCGTACAAGTGAGTGGCGGCCTACGCAACACGG
Internal bar code:GGGCTATATTATACTATACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1035
LEAP-Seq percent confirming:82.3529
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCACTGGAATGGTCACTC
Suggested primer 2:GTCAGTCCAGCTGCTTGACT