Insertion junction: CLIP2.021664_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.021664
Chromosome:chromosome 2
Location:6997668
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389541 OPR9 OctotricoPeptide Repeat protein 9; (1 of 1) IPR000104//IPR011237//IPR011335//IPR013584 - Antifreeze protein, type I // Peptidase M16 domain // Restriction endonuclease type II-like // RAP domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGCCGTTGGCGGCCCACTGCGGGTAGGCCAACTCTAGGAAGTCCAACA
Internal bar code:AGTCGGACCGAGGTCGCCTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1123
LEAP-Seq percent confirming:88.0952
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GATTTGGGTCTGCAGACGGA
Suggested primer 2:CGTGAAGGACATGACCACCA