| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.021666 |
| Chromosome: | chromosome 2 |
| Location: | 6819530 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388245 | (1 of 2) PF09811 - Essential protein Yae1, N terminal (Yae1_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCTGGTGAGGGCGCGGCCGGGGTGGTTCGGATTCGGGCGCCGCCGC |
| Internal bar code: | GTACCTAATGTAGAATGGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1656 |
| LEAP-Seq percent confirming: | 94.7917 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCGGGCTGAGTAATGTA |
| Suggested primer 2: | TAGACATCTTCCGCCGCATC |