| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021668 |
| Chromosome: | chromosome 14 |
| Location: | 1824603 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619750 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATTGGGAAGCCGCATTGCGAAAGTGCTTTGGCAGACTGGACCAAAGCA |
| Internal bar code: | AAGTAATTCTTGCCGCCACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3505 |
| LEAP-Seq percent confirming: | 84.3137 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAACTCCAGCAGTTCGTC |
| Suggested primer 2: | TGGACTTGAGCACAGTCGAC |