| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021676 |
| Chromosome: | chromosome 2 |
| Location: | 2534607 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092350 | CYP51G1,CYP6 | (1 of 1) 1.14.13.70 - Sterol 14-alpha-demethylase / Obtusufoliol 14-demethylase; Cytochrome P450, CYP51 superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACTGACTGGACCGGATGTTGGCTATGTATCCGTTGTCTGTCAAAGGCC |
| Internal bar code: | TACAGGGCCGGCATGCATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCGGACTTCTCAAGTTC |
| Suggested primer 2: | GCCTCAACATACCCAGCAGT |