| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.021679 |
| Chromosome: | chromosome 5 |
| Location: | 2174570 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237283 | (1 of 7) IPR020850 - GTPase effector domain, GED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCCGTTCCCCTGAAACCTTTCCCCACACCCCCTCCCCTTCAGCCCC |
| Internal bar code: | TCCGTAGGTTCCGCCGCCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1710 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGCATCGATGAGCTTTG |
| Suggested primer 2: | AGGGCAAGCTTAATGGGCAT |