Insertion junction: CLIP2.021689_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.021689
Chromosome:chromosome 3
Location:3163469
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGTCACACGCTCGTCATGACTGCAGCTTCGTCCAGGGGGGCTAGCTCC
Internal bar code:GTTTGAATCAAAGCCTACTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1078
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:TACAGGAACAAGACACGGGC
Suggested primer 2:TGCGCCTCGTGTATGATCTC