| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.021698 |
| Chromosome: | chromosome 9 |
| Location: | 701425 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404503 | (1 of 3) K16489 - protein SFI1 (SFI1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTGAGCGGCAACAGGCAGCCGCACGCCGGGCGCCACGAGCTGGGGTA |
| Internal bar code: | CATCAGTAACTATGGTGCAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5023 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTCCAGCAGTGAAGCAG |
| Suggested primer 2: | ACTCCGCCTTAACTGTGTCG |