| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.021700 |
| Chromosome: | chromosome 10 |
| Location: | 100279 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418250 | BLD10 | Basal body protein; (1 of 4) PTHR13140:SF289 - UNCONVENTIONAL MYOSIN-XIX | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCTGCGGCAGCAACTGGCTGCGGCGCGGGGCGAGCTGGCAGCCAGC |
| Internal bar code: | GATAATGGGCGTTGTATGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1105 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGATGAGTGGCAGAGGA |
| Suggested primer 2: | GTGTGCCAAACGAAAGCCAT |