Insertion junction: CLIP2.021712_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021712
Chromosome:chromosome 16
Location:2402013
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g659650 (1 of 100) IPR001810 - F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTCGGATCACATTCGCTTCATGCCGGGTGCGCGTGCTCAGGCACGGC
Internal bar code:ATAAAAAATTTTAAGCCAACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:136
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTCAACCATGGACCCTC
Suggested primer 2:CATCTAGGCTTGGGCTCAGG