Insertion junction: CLIP2.021721_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.021721
Chromosome:chromosome 3
Location:4964999
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179300 SRH6 (1 of 1) K11367 - chromodomain-helicase-DNA-binding protein 1 [EC:3.6.4.12] (CHD1); SNF2-related DNA/RNA helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGGACCACCACCCAGCCAACCCGCCCCCGTGACGCCGCACCCAACAC
Internal bar code:TAGAGAAGTCGGTTGTTTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:500
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCAGAGTACTCCCTGGAG
Suggested primer 2:GGAGAAGCGAGTGTGTGTCA