| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021733 |
| Chromosome: | chromosome 7 |
| Location: | 1359375 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322700 | CSA2 | Chlorophyceae-specific gene family A protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGAAGCCGCAGCTGCGGCGCCAGGTGAACCTGCTTCTAGCGGCCCCCA |
| Internal bar code: | CGCGATACACCTATTTATCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3830 |
| LEAP-Seq percent confirming: | 75.7143 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGCTTATTACGTGCGTGT |
| Suggested primer 2: | GCCACTTTCTGGATCCAGCT |