Insertion junction: CLIP2.021734_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.021734
Chromosome:chromosome 7
Location:2564485
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g329900 COP8,HKR4,COP (1 of 1) PF00072//PF00211//PF00512//PF01036//PF02518//PF07647 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // His Kinase A (phospho-acceptor) domain (HisKA) // Bacteriorhodopsin-like protein (Bac_rhodopsin) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) // SAM domain (Sterile alpha motif) (SAM_2); Histidine kinase rhodopsin 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGCTGCTCTACTTCTTCACGGCCCCCGCCATCATCCACATCCTGAGC
Internal bar code:CAGGCCGCCGATACATTCTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1769
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAGTGGCTCATTGGCTCA
Suggested primer 2:GCAGCTCACAGACAAAGCAC