| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021745 |
| Chromosome: | chromosome 4 |
| Location: | 1281721 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214750 | VMP7,VMPL2 | (1 of 2) PTHR21136:SF86 - VESICLE-ASSOCIATED MEMBRANE PROTEIN 7; Endosomal R-SNARE protein, VAMP-like family (R.III) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCAAGAACCGCGGGAAAGGCGCGGAATGACAGGAAGCTGAGGTGTCA |
| Internal bar code: | AGACTTGCGTAAAGGATAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1215 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGAGCCGCGTTCAGTATA |
| Suggested primer 2: | AGCCGAGAAAGCCTGTCTTC |