Insertion junction: CLIP2.021747_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.021747
Chromosome:chromosome 12
Location:5149550
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g526700 RBL9 Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAAACGGGTTGGGCAGCTGCGGCGGACCCGTGGAGGTGGCGGCAGCCA
Internal bar code:GTTTGGATCGGGGGGTTGTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1665
LEAP-Seq percent confirming:98.2456
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTGCTGACGGAAATCGCG
Suggested primer 2:GTTGTGCGTGTGTGTCTTCC