Insertion junction: CLIP2.021750_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021750
Chromosome:chromosome 13
Location:3843095
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAATGGCACGGAGATCCCGGTGCCGCTCAAGCGCCGCGACGTGTCTGC
Internal bar code:AGTGTTCACACTTACATATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1700
LEAP-Seq percent confirming:97.9798
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:99

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAGGACGTAGGTCACCT
Suggested primer 2:CTGGTGGACGAGGGACTAGA