| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021753 |
| Chromosome: | chromosome 6 |
| Location: | 1603968 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261450 | High mobility group protein; (1 of 1) K10802 - high mobility group protein B1 (HMGB1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGAGCCGTCTAGTCCCGCCTCGCCTTGGCCCCAGCCCAGCCCCAGCC |
| Internal bar code: | GTTTGACCTGTCCGCCTCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2837 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 170 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 170 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTGTGTTCTTCCGCTGG |
| Suggested primer 2: | AGCATGCATACCTTGGCCTT |