| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021782 |
| Chromosome: | chromosome 3 |
| Location: | 3964000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171300 | GYX1 | (1 of 1) 1.1.3.15 - (S)-2-hydroxy-acid oxidase / Hydroxy-acid oxidase B; Glycolate oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGAGTATCGCCGTAGCGCGTGGCCGCTCACAGCACATCACTGCAGGC |
| Internal bar code: | GACGGTAACGGGGGAGCACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5984 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 148 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 148 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGTGGAGAAGGTGAAGG |
| Suggested primer 2: | CACACAACGAAACAGGCCTG |