| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021793 |
| Chromosome: | chromosome 1 |
| Location: | 6497589 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045903 | DGAT1 | (1 of 1) K11155 - diacylglycerol O-acyltransferase 1 (DGAT1); Diacylglycerol O-acyltransferase, Type 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCAAGCTAAGACGAGCGGAGCACAGGCAGAGGCGTTAGTTGCAAGCGA |
| Internal bar code: | GCCAGTCATTGTTCATCAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGGCATTGAACCTGGG |
| Suggested primer 2: | ACCGCGAGTTCTACAAGGTG |