| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021813 |
| Chromosome: | chromosome 12 |
| Location: | 5441841 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529600 | RPA1 | DNA-directed RNA polymerase I subunit 1; (1 of 1) K02999 - DNA-directed RNA polymerase I subunit RPA1 (RPA1, POLR1A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACACTAGCTGCTCGTGCACACGATCAACACGTGCAGGTGGTGACAAG |
| Internal bar code: | CTAGCTGCGTGGAGCTAACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5657 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCACCTGCCCCCTCATTT |
| Suggested primer 2: | TCAGCCAGCTCTTTGGACTG |