Insertion junction: CLIP2.021816_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.021816
Chromosome:chromosome 13
Location:3849813
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTACATGCAAACGAGCGCTCAAGCTTTAAGCAAAACACAACTCGCTT
Internal bar code:CTGAGTGAGGGCGGTCCATCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:5481
LEAP-Seq percent confirming:96.5909
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGAAACAAATGTGCCCC
Suggested primer 2:AAAAACGTTCTCGCACACGG