| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021822 |
| Chromosome: | chromosome 12 |
| Location: | 4325973 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518900 | AKR9 | (1 of 4) 1.1.1.65 - Pyridoxine 4-dehydrogenase / Pyridoxine dehydrogenase; Aldo/keto reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTATAAGCGCATGTTTCTTCCCTTGCTGCTCTCTAACACAGCCCGAGC |
| Internal bar code: | GCGACAATGCAAGGGGAGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3863 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGCAGGTGTTCACAAAG |
| Suggested primer 2: | ACGGTTTGCGGATGTTACCT |