Insertion junction: CLIP2.021832_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.021832
Chromosome:chromosome 6
Location:1910749
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g264250 FAO6 FAD-dependent oxidoreductase; (1 of 3) 5.5.1.19 - Lycopene beta-cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGCAACAACACCACTGCCGACGTCATCCTCACCAACACGCCGCTGCA
Internal bar code:TAAGGTTATATACTTTAAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1785
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGGAGGCTGCACAAGGTG
Suggested primer 2:TGCAGTCTGGACGTGAAGAC