| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.021855 |
| Chromosome: | chromosome 14 |
| Location: | 3154990 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628600 | CYA13 | Putative adenylate cyclase; (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCACGTGTTGTGTGTGGACATGATGGAGGGTGCGTGTTTCGTGTGTGT |
| Internal bar code: | AAGGTAATGTATAAAGACTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2581 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCTGGGAAGCAAGTCA |
| Suggested primer 2: | GCTTATGGACGGCTCCTACC |