Insertion junction: CLIP2.021876_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021876
Chromosome:chromosome 12
Location:8472826
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g549750 FAP4,CK1,CSK1 Flagellar Associated Protein 4; (1 of 2) K02218 - casein kinase 1 (CSNK1, CK1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGCTTAAAAACCGGACATATACAACCTAGTAGAAGCACTGCTCATAG
Internal bar code:GACTCGATGGGACACTAAATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2575
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACCGACTCCTGCAAGTC
Suggested primer 2:TTCATCCGCATGTCCAGCTT