| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021876 |
| Chromosome: | chromosome 12 |
| Location: | 8472872 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549750 | FAP4,CK1,CSK1 | Flagellar Associated Protein 4; (1 of 2) K02218 - casein kinase 1 (CSNK1, CK1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCGGGGGCTGCGGCATCGGGGGCTGCATCGGGGGCCGTTTTGTCCT |
| Internal bar code: | TTCTTGAACAGATGCGAGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2368 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATCCGCATGTCCAGCTT |
| Suggested primer 2: | TTGACCGACTCCTGCAAGTC |