Insertion junction: CLIP2.021877_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021877
Chromosome:chromosome 10
Location:2040169
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g432500 CND1 Condensin complex component; (1 of 1) K06677 - condensin complex subunit 1 (YCS4, CNAP1, CAPD2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCAACCTGGCGCTAATCACCTCGCGCACTAACGTGGCGGCCATGATGC
Internal bar code:ACTAGTGTAGCTATCGTAATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2723
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCACCCCGTCAGGCTAC
Suggested primer 2:GCATGGACGCAATCGAGTTC