Insertion junction: CLIP2.021888_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.021888
Chromosome:chromosome 4
Location:1722122
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g212200 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCTCTGCGTAGCGTACAGGTGCTGCCAGGACCTGGCGAGGTGCTGAAG
Internal bar code:CATTCCAGTGAGCAACGCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:850
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTCTGCTGACGTGTTGC
Suggested primer 2:CAGACACCGCTTTCAGCAAC