| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021896 |
| Chromosome: | chromosome 1 |
| Location: | 6721713 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048050 | COA3,COAB1,PPCS1 | (1 of 1) 6.3.2.5 - Phosphopantothenate--cysteine ligase / Phosphopantothenoylcysteine synthetase; Phosphopantothenate-cysteine ligase, CoA biosynthesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGAGGGCGCACCGTGTTGCCCAATGACGCAGTCTCTAGCAATGGTCC |
| Internal bar code: | ATGACGCAGTTAGAGTAAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 6.25 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGTGACCCTACATGTC |
| Suggested primer 2: | CAGACGCATGCATGTCTTCG |