| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021897 |
| Chromosome: | chromosome 1 |
| Location: | 1993638 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010900 | GAPA1,GAP3 | (1 of 1) 1.2.1.13 - Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) / Triosephosphate dehydrogenase (NADP+); Glyceraldehyde-3-Phosphate Dehydrogenase A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGGTGACAAGTCGCTGGTCTTTGGATATGTATTGCTGTTCGGTATT |
| Internal bar code: | GGCCCCCGTCATTAGTTAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2059 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGTCACGCAGTTCCCAA |
| Suggested primer 2: | GACACCAGTGCCCTCAATGA |