| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.021912 |
| Chromosome: | chromosome 3 |
| Location: | 5227538 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGGCGGGAGGTCGATTGCGGCATATTGCGGCTTGGACGCACGTGGCC |
| Internal bar code: | CAGGTCGTGATATCGGGAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2120 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCTCAGTTCCTGCTGA |
| Suggested primer 2: | AAACAATCACCAACGCAGCC |