Insertion junction: CLIP2.021914_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.021914
Chromosome:chromosome 10
Location:496003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421050 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATGGTCGGCCGCGGTTGGTAATGCGATCTCGGACGTCACGCGCCACTG
Internal bar code:ACCCGGATCTTGGATGTGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4232
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:ATCACCACACGCCTGCTTTA
Suggested primer 2:ATTGCCAAGGCCGTCAAAAC