| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021954 |
| Chromosome: | chromosome 11 |
| Location: | 543237 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467599 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTCGACAGCTGTAGTTTATGCGACTGAGGAGCGTAGCACGATCCGCT |
| Internal bar code: | TCTGTGTATTTCTGCTAAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1370 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACTCACTTGCCGAACCA |
| Suggested primer 2: | CGGATGATGCAGTAGGGGTC |