| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.021975 |
| Chromosome: | chromosome 8 |
| Location: | 1907795 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367800 | NDH2 | Nucleoside diphosphate hydrolase; (1 of 1) 3.6.1.13//3.6.1.22 - ADP-ribose diphosphatase / ADPR-PPase // NAD(+) diphosphatase / NADP pyrophosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCTGCAAGCCTGCTAGGTGCCTTGCATCTCGTCCAACAGTCCTCAT |
| Internal bar code: | ATTGCGGAATCGTTAGGGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1245 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGATGGGAGAGAAGGAGT |
| Suggested primer 2: | GGATAACGATGTCCGGGTCC |