| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021984 |
| Chromosome: | chromosome 12 |
| Location: | 1939603 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511640 | PIGX,PIGX1 | (1 of 1) PF08320 - PIG-X / PBN1 (PIG-X); co-factor of alpha-(1-4)-Mannosyltransferase, PIG-X | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGTGGTACTGGTGTGATGTGATGGCAGAAATGGCTCGGCGAGGCGTG |
| Internal bar code: | AGTGCGGGGTTCCTGACTCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1258 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAGGTGCGTTATGGCTT |
| Suggested primer 2: | CACAGCCCAACCCAACATTG |