| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.021990 |
| Chromosome: | chromosome 13 |
| Location: | 3481711 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587500 | AOF8 | (1 of 2) 1.3.5.5 - 15-cis-phytoene desaturase / Plant-type phytoene desaturase; Flavin-containing amine oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGGTTCCAGAACCTGTGACGGATGCAAAATGGTGTTGCACCGCTTG |
| Internal bar code: | TAGATGCATCTGGGGGTCTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTATCAGGTGAGGGAGGG |
| Suggested primer 2: | CTGACCGTATGCGCTCATCT |