Insertion junction: CLIP2.022016_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.022016
Chromosome:chromosome 13
Location:3543926
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g587750 COG6 Component of oligomeric golgi complex; (1 of 1) PF06419 - Conserved oligomeric complex COG6 (COG6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAAGCCTCCGGCCACCCCCAGCCCCAGCCTCGGCCCCCCTCCCCTCAC
Internal bar code:CGTGGTTCAGCTAATGCCACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:462
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCCAGTCAGGAAGATGTC
Suggested primer 2:ACACACACACACACACCCAT