| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.022064 |
| Chromosome: | chromosome 1 |
| Location: | 3998713 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026250 | AGA1 | Alpha-galactosidase; (1 of 1) 3.2.1.22 - Alpha-galactosidase / Melibiase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGCGCTGTCCTAGCACACTCACACATGTCCTCGTTCAAACACACAC |
| Internal bar code: | CTGAACATGTTAACATTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1397 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGTTCATGTCGCTGCAT |
| Suggested primer 2: | GCCCGTTGTGTTTGACAGAC |