| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.022079 |
| Chromosome: | chromosome 7 |
| Location: | 1473570 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323950 | PFH23,PHX23 | Putative prolyl 4-hydroxylase; (1 of 1) PTHR10869//PTHR10869:SF50 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACCGCGAGACATACGCGACCGACACATTGCTGTGAAAGGGTGTCAAG |
| Internal bar code: | GAGGACGTCGGGAAGTACTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1192 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCAACAGTTAGCAGCGT |
| Suggested primer 2: | CCATTGGCGTTTGTACCTGC |