Insertion junction: CLIP2.022095_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.022095
Chromosome:chromosome 12
Location:3300101
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g499000 (1 of 1) PF03879 - Cgr1 family (Cgr1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAACGGCGCCCCTCAGGGGGCGACCAGACACCAAGCGCGGGACACTCC
Internal bar code:ATGGTGTGACATTATACAGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:5233
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCAATCTCCACGACGCT
Suggested primer 2:GTCATCCTCCTAAGCGCCTC