| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.022103 |
| Chromosome: | chromosome 10 |
| Location: | 6074917 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461900 | AKR8 | Aldo/keto reductase; (1 of 2) PTHR11732:SF255 - ARYL-ALCOHOL DEHYDROGENASE YPL088W-RELATED | 5'UTR |
| Cre10.g801204 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAACCGGGCAGCGGCGGCAAGACCTGGCTGGCCCACCAACATGCCTGT |
| Internal bar code: | TAAGGCGTAGGGCACTCGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1199 |
| LEAP-Seq percent confirming: | 74.1936 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 124 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGAGCTCAACAAAGTTGC |
| Suggested primer 2: | GGTTTCGCGAGCCTTGTTTT |