Insertion junction: CLIP2.022109_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022109
Chromosome:chromosome 15
Location:1461132
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g641850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCAAGGGTGGTGCAGATTGCCGGTCCGCATGCACACAATCGCAAGGAA
Internal bar code:TTAGATTTTCGATCAACGGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTTGTTGTTGTTGTTGC
Suggested primer 2:AAAAGTGTTTCCGACGCGTG