| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022112 |
| Chromosome: | chromosome 12 |
| Location: | 3612997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513150 | MIA40 | (1 of 1) K17046 - protein DEK (DEK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCAAGACAAAACACAACCCGAACGTTCGAACTAGCCAGCAGCAACC |
| Internal bar code: | TTGCGCATGTGAAGTCCCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4518 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 139 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCGAAGAAGGCGAAGAA |
| Suggested primer 2: | AGGGAGTCCGTCACAGCTAT |