Insertion junction: CLIP2.022114_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.022114
Chromosome:chromosome 16
Location:2085327
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g657450 MPA12 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) 3.6.1.13//3.6.1.16//3.6.1.53 - ADP-ribose diphosphatase / ADPR-PPase // CDP-glycerol diphosphatase / CDP-glycerol pyrophosphatase // Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase / Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCACTGCAATGAGTTCACCTGCCCTGGCGTCAGGTCCCACCGCCGCAC
Internal bar code:GCAGCGATGTCGGTCATATTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2075
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATCACAGATCGAGGCTC
Suggested primer 2:CTCACCAACGTGCAAACAGG