Insertion junction: CLIP2.022120_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.022120
Chromosome:chromosome 13
Location:1640780
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCCATGAAACTTGATGGGCGCACTGACACACGCACGCCGTCCGCGCAA
Internal bar code:CTTGTGGCGAGGCAATACAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2371
LEAP-Seq percent confirming:73.4694
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCCGTTCTTGAAGTCCT
Suggested primer 2:ATCGGAACTGCAGCACATCA