Insertion junction: CLIP2.022124_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.022124
Chromosome:chromosome 1
Location:190259
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g001300 ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGTAAAAGCACACACACACACACACACACACACACACACACAGCCACC
Internal bar code:TGGTCGACTGTTAGTGTAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:666
LEAP-Seq percent confirming:58.3333
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCAGATCCAGTTCACTC
Suggested primer 2:AACATGGTTCAACACGCACG