| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022134 |
| Chromosome: | chromosome 1 |
| Location: | 3980223 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026050 | protein of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCCTGGTGGTTCAACATAGTGTATGTATAGTGGGCGTAGAACCTAC |
| Internal bar code: | TTCAACCGAATATACGGTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1808 |
| LEAP-Seq percent confirming: | 90.6977 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAGTAGAGTCGAGGGCA |
| Suggested primer 2: | ATGTTGGGACGCTGGACATT |