Insertion junction: CLIP2.022147_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.022147
Chromosome:chromosome 7
Location:5511935
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g351400 MCM10 (1 of 1) PTHR13454//PTHR13454:SF11 - FAMILY NOT NAMED // PROTEIN MCM10 HOMOLOG; Minichromosome maintenance protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACACACGCAAACGCACGCACACAAAAACACACAGTTTCCTTTCCTTGT
Internal bar code:GATCGGATTCGAGAACAACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2342
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCACCCTGTCGGTTTTTG
Suggested primer 2:CGAGCTCAAACAATCCCCCT