| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.022153 |
| Chromosome: | chromosome 6 |
| Location: | 7280826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGGCCATGGACAGAGGCGGCGCATGCGGCATGGCGTGTGCCGCGAT |
| Internal bar code: | CTTTTGTGTAAGTGTCCCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 70.2128 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGAGTTTGACGAGGAGG |
| Suggested primer 2: | GGGTATTCGCCGTGCTCATA |